NTR expression in ESCC cell line. p75NTR+ cells made up 2.5% in Eca109 (A), 1.6% in SHEC-1 (B), 1.9% in SHEC-4 (C) and 3.7% (D) in SHEC-5. Cells derived from p75NTR+ cells contained both p75NTR+ and p75NTR- cells, whereas those from p75NTR- cells generated only p75NTR- cells. In p75NTR+ subpopulation, the percentage of p75NTR+ cells declined time-dependently. In addition, p75NTR+ cells could be serial passaged and generated p75NTR+ β1-integrin-, p75NTR- β1-integrin-and p75NTR- β1-integrin+ progenies (E), whereas p75NTR- cells generated only p75NTR- cells in 5th passage (F).