Figure 2

p75 ^NTR expression in ESCC cell line. p75^NTR+ cells made up 2.5% in Eca109 (A), 1.6% in SHEC-1 (B), 1.9% in SHEC-4 (C) and 3.7% (D) in SHEC-5. Cells derived from p75^NTR+ cells contained both p75^NTR+ and p75^NTR- cells, whereas those from p75^NTR- cells generated only p75^NTR- cells. In p75^NTR+ subpopulation, the percentage of p75^NTR+ cells declined time-dependently. In addition, p75^NTR+ cells could be serial passaged and generated p75^NTR+ β1-integrin^-, p75^NTR- β1-integrin^-and p75^NTR- β1-integrin⁺ progenies (E), whereas p75^NTR- cells generated only p75^NTR- cells in 5th passage (F).