Overexpression of UbcH10 promotes the cellular proliferation and alters the cell cycle profile in colon cancer. (A) UbcH10 overexpression in DLD1 colon cancer cells. (B) Cellular proliferation was markedly increased in UbcH10-overexpressed DLD1 cells compared with control cells. (C) Results of anchorage-independent colony formation soft-agar assay. The number of colonies was markedly increased in stably-transfected UbcH10 DLD1 colon cancer cells. (D) Cell cycle profile of overexpresed UbcH10 and control DLD1 cells shows that the population of G2/M cells is moderately decreased with the delivery of UbcH10. (E) a. Immunofluorescence with phosphorylated-histone H3 (pH3). The population of pH3 positive cells was decreased in UbcH10-overexpressed DLD1 cells. b. Quantification of immunofluorescence analysis (triplicate experiments).