Figure 6

Inhibition of Src or STAT3 leads to PARP cleavage and downregulates survivin expression in OSA cell lines. A) Canine or B) human OSA cell lines were treated for 24 or 48 hours with DMSO, SU6656, or LLL3. Cells were collected and protein lysates were separated via SDS-PAGE. Western blotting for survivin, PARP and β-actin was performed. The anti-PARP antibody utilized in our laboratory has been applied to experiments with canine cell lines and recognizes a 113 kDa intact PARP protein and a 23 kDa cleaved PARP fragment [53].