Figure 6

Human metastatic liver tumors of primary colorectal adenocarcinomas expressed C2-O-sLe ^x. (A) Photomicrograph of a tissue section of metastatic colorectal adenocarcinoma tumors in liver stained with hematoxylin and eosin, scale bar = 150 μm. (B) A serial section of the same tissue as shown in (A), stained with CHO-131 mAb (15 μg/ml). Red color indicates positive reactivity with the CHO-131 mAb, scale bar = 150 μm. (C) A different tissue section of metastatic colorectal adenocarcinoma in liver stained with CHO-131 mAb, scale bar = 100 μm. (D) A higher magnification of the same tissue section as in (C), stained with CHO-131 mAb (15 μg/ml), scale bar = 50 μm. (E) A serial section of the same tissue as shown in (C) and (D), stained with CEA mAb (1.6 μg/ml) as a positive control. Brown stained cells are positive for CEA mAb, scale bar = 100 μm. (F) Normal liver stained with CHO-131 mAb (15 μg/ml). Note the absence of red color, indicating a lack of reactivity with CHO-131 mAb, scale bar = 100 μm. All tissue sections were 4 μm thickness. Figures (A) and (B) 100× magnification, (C) 200× magnification, (D) 400× magnification, (E) and (F) 200× magnification. Mayer's hematoxylin was used as a counterstain for tissues stained with CHO-131 and CEA mAbs. Representative sections from multiple stained tissues are shown.