E- and P-cadherin modify the migratory and invasive behaviour of 231 cells. A: Study of cell migration by wound healing assay. Left pictures show that control cells migrate as single cells (arrow) whereas 231_E-cadh and 231_P-cadh cells migrate as clusters. Right: Cell migration was quantified by measuring the wounded area (in pixels) that was covered by the cells in the indicated time points. Each bar represents mean ± SEM of four independent experiments. B: Invasion ability through extracellular matrix (Matrigel) assessed by Transwell assays. Left: Representative images showing the number of invaded cells (nuclei stained by DAPI) after 24 hours. Right: Quantification of invasion. Each bar represents mean number ± SEM of invaded cells for each condition (four independent experiments). Mean differences were compared by Student's t test (* indicates differences statistically significant p < 0.05; NS, not significant).