Figure 3
Altered genomic dosage of TRIM3 suggests homozygous deletions in human primary gliomas. A. Genomic dosage of the TRIM3 gene area of ASIII 098, GBM 157, and GBM 164 as determined by quantitative real-time PCR. Light grey background indicates ΔCt cut-offs for diploidy (2n = 0 ± 0.3) and haploidy (n = -1 ± 0.3) for each primary tumor sample; grey bars depict ΔCt values of amplified tumor DNA related to a structural map of the TRIM3 gene with coding regions shown in black B. Mapping of indicated homozygous deletions within the TRIM3 gene. STS-based and SNP-based compilation of Q-PCR data is shown in relation to structural map of the TRIM3 gene with coding regions shown in black. Tumor histology, grades and numbers are indicated on the left. STS-based (D11S series) allelic loss and non-informative data are represented by closed and grey circles, respectively. Areas of local allelic retention defined by SNP analysis (rs series) within segments of allelic loss defined by STS analysis are framed by open rectangles. Copy number deduced from Q-PCR data are indicated; minimal and maximal extension of allelic loss' are shown with filled and hatched grey areas, respectively; areas of homozygous loss are shown in black. Q-PCR data indicate homozygous deletions in primary tumors ASIII 098 and GBM 157. Abbreviations: GBM, Glioblastoma multiforme; AS, astrocytoma; OG, oligodendroglioma; RET, retention; LOH, loss of heterozygosity; NI, non-informative; Q-PCR, quantitative real-time polymerase chain reaction.