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Figure 3 | BMC Cancer

Figure 3

From: ARC (NSC 188491) has identical activity to Sangivamycin (NSC 65346) including inhibition of both P-TEFb and PKC

Figure 3

ARC perturbs the cell cycle and induces apoptosis in a similar manner to sangivamycin and toyocamycin. (A) HL60 cells were incubated with 2 μM of each drug for 48 h and assessed for cell cycle block by flow cytometry using propidium iodide (left panel) or for apoptosis using propidium iodide (Y axis) and Annexin V-Alexa488 (X axis) (right panel). (B) ARC and sangivamycin have similar effects on RNA, DNA and protein synthesis. Cells were incubated with varying concentrations (1–10000 nM) of ARC, sangivamycin, toyocamycin, fludarabine and thioguanine for 3 to 24 h. Shown is one of three representative experiments depicting the incorporation of [3H] uridine into RNA, [3H] thymidine into DNA and [14C] leucine into protein after exposure of MCF7 cells to 1000 nM of the different adenosine analogs for 3 h (upper panel) or 6 h (lower panel) as a % of control treated cells.

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