Figure 1

Generation of ROS during bortezomib treatment. A, Cells were treated with the indicated concentration of bortezomib for 24 hours and apoptotic cells were analyzed. B, Cells were treated with 50 nM bortezomib for the indicated time, apoptotic cells were analyzed using annexin V/PI double staining and subsequent flow cytometry. C, Cells were treated with 50 nM bortezomib for 8 hours and intracellular caspase 3 activity was measured. D-E, Cells were treated with 50 nM bortezomib for the indicated time, proteasome activity (D) and production of ROS (E) were analyzed. *, P< 0.05; ** P < 0.001 vs vehicle (A, C) or 0 hour treatment (B, D-E).