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Table 3 Features of neural and mesenchymal differentiation presented by glioblastoma cells

From: Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors

fg GBM1 GBM2 GBM3 GBM4 GBM8 GFAP+NNP
p0
GFAP+NNP
p1
Aggregated
GBM cells with multilineage phenotype
100 100 100 100 100 no no
5d Multilineage 100 100 95–100 100 83–87 0–1 0–1
5d NI 0 0 0–2 0 3–6 1–2 1–2
5dMES 0 0 0–2 0 3–5 0 5–8
5dGLIA 0 0 0–3 0 6–10 44–46 45–49
5dNEURO 0 0 0 0 0–3 54–56 47–50
5d not classified 0 0 0–2 0 3–5 0–1 0–1
20d Multilineage 19–23 72–75 12–15 42–46 18–22 0 0
20dNI 21–25 8–12 23–25 11–15 16–20 0 0
20dMES 26–29 3–6 36–40 7–11 4–6 0 5–8
20dGLIA 21–24* 4–9* 15–19* 25–31* 44–51* 48–50* 45–44*
20dNEURO 0 0 0 0 5–7* 50–52* 48–50*
20d not classified 9–12 8–11 8–13 9–12 9–11 0–1 0–1
  1. GBM, glioblastoma;
  2. GFAP+NNP, normal neural progenitors;
  3. NI, neuronal intermediates;
  4. MES, mesenchymal cells;
  5. GLIA, glial cells;
  6. NEURO, neuronal cells;
  7. 5d, five days of differentiation (DMEM/F12 N2);
  8. 20d, twenty days of differentiation (DMEM/F12 N2);
  9. no, GFAP+NNP did not show stable aggregates.
  10. *After 5 and 20 days of exposure to the differentiation medium glioblastoma cells showed Nestin expression. GFAP+NNP derivatives showed Nestin expression after 5 but not after 20 days of serum starvation.