Table 1 Primary antibodies used for Western blot (WB) and immunocytochemical staining (IC)
I Ab | Host | Manufacturer | Application (Dilution) |
|---|---|---|---|
anti-nestin | mouse | Santa Cruz Biotechnology, Inc.; sc-23927 | WB (1:200); IC (1:100) |
anti-GFAP | mouse | Chemicon; MAB360 | WB (1:800); IC (1:400) |
anti-CD44 | mouse | Santa Cruz Biotechnology, Inc.; sc-7297 | WB (1:100); IC (1:100) |
anti-MAP-2 | rabbit | Santa Cruz Biotechnology, Inc.; sc-20172 | WB (1:200); IC (1:100) |
anti-nestin | rabbit | Santa Cruz Biotechnology, Inc.; sc-20978 | IC (1:100) |
anti-CD133 | rabbit | Santa Cruz Biotechnology, Inc.;sc-30220 | IC (1:100) |
anti-ÎČIII-tubulin | rabbit | Sigma; T 2200 | WB (1:250); IC (1:250) |
anti-Fibronectin | rabbit | Sigma; F 3648 | IC (1:200) |
anti-SOX2 | rabbit | Chemicon; AB5603 | IC (1:1000) |
anti-GFAP | goat | Santa Cruz Biotechnology, Inc.; sc-6171 | IC (1:50) |
anti-vimentin | goat | Chemicon; AB-1620 | WB (1:100); IC (1:40) |
anti-ÎČIII-tubulin | mouse | Chemicon MAB 1637 | IC (1:200) |
anti-TH | mouse | Santa Cruz Biotechnology, Inc.; sc-25269 | IC (1:100) |
anti-BrdU | mouse | Sigma B 8434 | IC (1:500) |