Figure 4

AZD0530 inhibits survival signalling pathways involved in Bcr-Abl leukaemogenesis. Whole cell lysates from AZD0530 and Imatinib treated BV173 cells (left) and SEM cells (right) were probed with antibodies (phosphorylated and non-phosphorylated forms) against Akt, Stat5 and Erk. Specificity of both inhibitors to Bcr-Abl was tested by comparing results of BV173 and SEM cells treated under similar conditions. In BV173 cells the activation of Akt, Stat5 and Erk was inhibited by AZD0530 and Imatinib, in contrast to SEM cells which showed no regulation of all three proteins. One representative experiment out of 3 is given.