Figure 2

Apoptosis induction by AZD0530. A Inhibition of SFK and Bcr-Abl by AZD0530 and Imatinib leads to a Bcr-Abl-dependent induction of apoptosis, as seen in BV173 (Ph+) versus SEM (Ph-) cell lines. One representative experiment out of 3 is given. B Induction of apoptosis in WTSupB15 cells and not in RTSupB15 cells. All four cell lines (BV173 (Ph+), SEM (Ph-), WTSupB15 (Ph +) and RTSupB15 (Ph+ Imatinib resistant)) were treated with increasing concentrations of both compounds for three days. The percentage of apoptotic cells was measured by staining with 7-AAD. Results represent the mean values of three independent experiments +/- SD. For PARP cleavage, cells were treated for three days and immunoblotted. Intact (116 kDa) and cleaved (85 kDa) PARP are shown. Equal protein loading was monitored by probing tubulin on the same membrane. One representative experiment out of 3 is given.