Figure 3

CtBP1 splice product in melanoma cells. As observed in Fig. 1A a smaller CtBP1 splice variant is expressed in melanoma cells. (A) Using primers flanking the region coded by exon 4 revealed lack of exon 4 in the melanoma cell line Mel Im in comparison to normal human melanocytes (NHEM). The expression of CtBP1splice was shown in eight of eleven melanoma cell lines using PCR (B) Sequence analysis of the PCR product shown in (A) confirmed lack of the coding region represented by exon 4. (C) In a schematic drawing localization of exon 4 of CtBP1 is displayed.