Figure 1

(a) Representative RAPD gel profile showing alterations (arrows) in the form of loss/gain/change in intensity of band(s) in tumor (T) as compared to normal leucocyte DNA (B) of the same patient, with different primers (primer nos. 80/01, 70/04, 70/01, 60/31). (b-i) RAPD gel profile of primer no. 80/07 indicating the frequent loss of a 500 bp band (arrow) in astrocytic tumors (T) as compared to the corresponding normal leucocyte DNA (B). (b-ii) Southern blot of the same RAPD profile, Southern hybridization was done to confirm the altered fragment with a radiolabeled probe prepared from 500 bp altered band eluted from the RAPD profile of normal DNA from another gel. (c) Amplification of normal (B) and tumor (T) DNA with loss of 500 bp band with specific primer pair designed to amplify the FAT gene corresponding to the altered band along with 100 bp on either side. Bands were eluted from the gel and sequenced to look for deletion(s)/mutation(s) at RAPD primer binding sites. M represents molecular marker.