LN18 and LN229 cells express the MIF receptor complex proteins CD44 and CD74. FACS analysis revealed a strong expression of CD44 on both cell lines (A), which was confirmed by Western Blotting (B). Analysis of CD74 expression by FACS did not show a significant difference by flow cytometry (A), but strong expression of CD74 in the LN229 and weak expression in the LN18 cells could be detected by Western Blotting (C). Expression ratios for CD44 andCD74 relative to Actin have been calculated from three independent experiments. For FACS analysis filled area show autofluorescence of unstained cells and the thin graph represents the staining of the isotype control, whereas the signal of the PE labeled anti CD44 and FITC labeled anti CD74 is shown by the bold graph.