|  |
Methylation levels estimated for CDKN2A
|
Methylation levels estimated for RARB
|
---|
Sample
|
Immunohistochemistry for p16
|
MS-HRM
|
SMART-MSP
|
MS-HRM
|
SMART-MSP
|
---|
 |  |  |
Lowest estimate
|
Average estimate
|
Highest estimate
| Â |
Lowest estimate
|
Average estimate
|
Highest estimate
|
---|
1 (1978)
|
Negative
|
0.1%-1%
|
1.7%
|
2.7%
|
3.4%
|
Failed ampl
|
0%
|
0%
|
0%
|
2 (1978)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0.1%-1%
|
0%
|
0%
|
0%
|
3 (1978)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0%
|
0%
|
0%
|
4 (1978)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
1%-10%
|
2.4%
|
9.5%
|
30.8%
|
5 (1978)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
10%-100%
|
14.4%
|
20.3%
|
28.7%
|
6 (1978)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0.1%-1%
|
0.1%
|
0.3%
|
0.4%
|
7 (1978)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
8 (1978)
|
No data
|
10%-100%
|
20.3%
|
25.0%
|
28.7%
|
10%-100%
|
57.4%
|
61.6%
|
75.8%
|
9 (1983)
|
Negative
|
1%-10%
|
0.7%
|
1.9%
|
5.4%
|
Failed ampl
|
4.1%
|
5.8%
|
7.2%
|
10 (1983)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
10%-100%
|
21.8%
|
26.8%
|
33.0%
|
11 (1983)
|
Negative
|
0.1%-1%
|
1.7%
|
2.4%
|
3.3%
|
Het met
|
0%
|
0%
|
0%
|
12 (1983)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
1%-10%
|
18.9%
|
33.0%
|
53.6%
|
13 (1983)
|
Negative
|
1%-10%
|
19.0%
|
27.0%
|
30.8%
|
0%
|
0%
|
0%
|
0%
|
14 (1983)
|
Negative
|
0.1%-1%
|
0.8%
|
2.5%
|
6.3%
|
Het met
|
0.2%
|
0.4%
|
1.4%
|
15 (1983)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0.1%-1%
|
0.3%
|
0.9%
|
5.1%
|
16 (1988)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.4%
|
0.8%
|
1.3%
|
17 (1988)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
10%-100%
|
93.3%
|
114.9%
|
186.6%
|
18 (1988)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
Failed ampl
|
0%
|
0%
|
0%
|
19 (1988)
|
Negative
|
1%-10%
|
3.4%
|
5.8%
|
10.9%
|
Het met
|
0.2%
|
0.8%
|
3.8%
|
20 (1988)
|
Negative
|
10%-100%
|
12.5%
|
14.4%
|
21.8%
|
0%
|
0%
|
0%
|
0%
|
21 (1988)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.1%
|
0.5%
|
2.4%
|
22 (1988)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Het met
|
2.2%
|
2.7%
|
3.8%
|
23 (1988)
|
Negative
|
10%-100%
|
18.9%
|
20.3%
|
21.7%
|
0%
|
0%
|
0%
|
0%
|
24 (1988)
|
Negative
|
10%-100%
|
9.5%
|
14.4%
|
15.4%
|
Het met
|
0.1%
|
0.2%
|
0.7%
|
25 (1988)
|
Negative
|
10%-100%
|
6.3%
|
7.7%
|
10.9%
|
0%
|
0%
|
0%
|
0%
|
26 (1988)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.2%
|
0.3%
|
0.6%
|
27 (1988)
|
Negative
|
10%-100%
|
12.5%
|
14.4%
|
15.4%
|
Failed ampl
|
0%
|
0%
|
0%
|
28 (1988)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
29 (1993)
|
Negative
|
1%-10%
|
3.6%
|
5.8%
|
7.7%
|
0%
|
0%
|
0%
|
0%
|
30 (1993)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
1%-10%
|
2.9%
|
8.8%
|
14.4%
|
31 (1993)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.3%
|
0.7%
|
1.4%
|
32 (1993)
|
Negative
|
10%-100%
|
21.8%
|
26.8%
|
30.8%
|
0.1%-1%
|
0.1%
|
0.3%
|
0.7%
|
33 (1993)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
34 (1993)
|
Negative
|
10%-100%
|
25.0%
|
40.6%
|
61.6%
|
1%-10%
|
2.9%
|
5.4%
|
8.8%
|
35 (1993)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.3%
|
0.6%
|
0.8%
|
36 (1993)
|
Negative
|
10%-100%
|
5.1%
|
10.9%
|
17.7%
|
0.1%-1%
|
0.2%
|
0.6%
|
1.4%
|
37 (1993)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
1%-10%
|
23.3%
|
25.0%
|
28.7%
|
38 (1993)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
39 (1998)
|
Negative
|
0.1%-1%
|
0%
|
0%
|
0%
|
0%
|
2.5%
|
2.9%
|
3.4%
|
40 (1998)
|
Negative
|
0.1%-1%
|
0.1%
|
0.1%
|
0.2%
|
Het met
|
0.6%
|
1.5%
|
3.1%
|
41 (1998)
|
Negative
|
10%-100%
|
19.0%
|
23.3%
|
28.7%
|
0%
|
0%
|
0%
|
0%
|
42 (1998)
|
Positive
|
0%
|
0.1%
|
0.3%
|
0.7%
|
Het met
|
0.2%
|
0.4%
|
1.4%
|
43 (1998)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
44 (1998)
|
Positive
|
0.1%-1%
|
0.1%
|
0.2%
|
0.4%
|
Het met
|
2.9%
|
3.6%
|
5.4%
|
45 (1998)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0.1%-1%
|
0.2%
|
0.7%
|
2.9%
|
46 (1998)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0.1%-1%
|
0%
|
0%
|
0%
|
47 (1998)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
10-100%
|
21.8%
|
30.8%
|
50.0%
|
48 (1998)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Failed ampl
|
0%
|
0%
|
0%
|
49 (2003)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
50 (2003)
|
Positive
|
0%
|
0%
|
0%
|
0%
|
Het met
|
0.5%
|
1%
|
2.1%
|
51 (2003)
|
No data
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
52 (2003)
|
Positive
|
0.1%-1%
|
0.4%
|
0.6%
|
1.0%
|
1%-10%
|
2.7%
|
3.9%
|
5.1%
|
53 (2003)
|
Negative
|
10%-100%
|
2.7%
|
6.3%
|
16.5%
|
1%-10%
|
4.1%
|
10.2%
|
26.8%
|
54 (2003)
|
Negative
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
0%
|
- An average methylation estimate as well as the highest and lowest possible methylation estimates calculated from the Ct-values of the triplicates for the gene and the control is given for SMART-MSP.