Table 2 Methylation levels of the samples as estimated by MS-HRM and SMART-MSP.
Methylation levels estimated for CDKN2A | Methylation levels estimated for RARB | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Sample | Immunohistochemistry for p16 | MS-HRM | SMART-MSP | MS-HRM | SMART-MSP | ||||
Lowest estimate | Average estimate | Highest estimate | Lowest estimate | Average estimate | Highest estimate | ||||
1 (1978) | Negative | 0.1%-1% | 1.7% | 2.7% | 3.4% | Failed ampl | 0% | 0% | 0% |
2 (1978) | Negative | 0% | 0% | 0% | 0% | 0.1%-1% | 0% | 0% | 0% |
3 (1978) | Positive | 0% | 0% | 0% | 0% | Het met | 0% | 0% | 0% |
4 (1978) | Negative | 0% | 0% | 0% | 0% | 1%-10% | 2.4% | 9.5% | 30.8% |
5 (1978) | Positive | 0% | 0% | 0% | 0% | 10%-100% | 14.4% | 20.3% | 28.7% |
6 (1978) | Negative | 0% | 0% | 0% | 0% | 0.1%-1% | 0.1% | 0.3% | 0.4% |
7 (1978) | Negative | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
8 (1978) | No data | 10%-100% | 20.3% | 25.0% | 28.7% | 10%-100% | 57.4% | 61.6% | 75.8% |
9 (1983) | Negative | 1%-10% | 0.7% | 1.9% | 5.4% | Failed ampl | 4.1% | 5.8% | 7.2% |
10 (1983) | Positive | 0% | 0% | 0% | 0% | 10%-100% | 21.8% | 26.8% | 33.0% |
11 (1983) | Negative | 0.1%-1% | 1.7% | 2.4% | 3.3% | Het met | 0% | 0% | 0% |
12 (1983) | Positive | 0% | 0% | 0% | 0% | 1%-10% | 18.9% | 33.0% | 53.6% |
13 (1983) | Negative | 1%-10% | 19.0% | 27.0% | 30.8% | 0% | 0% | 0% | 0% |
14 (1983) | Negative | 0.1%-1% | 0.8% | 2.5% | 6.3% | Het met | 0.2% | 0.4% | 1.4% |
15 (1983) | Negative | 0% | 0% | 0% | 0% | 0.1%-1% | 0.3% | 0.9% | 5.1% |
16 (1988) | Negative | 0% | 0% | 0% | 0% | Het met | 0.4% | 0.8% | 1.3% |
17 (1988) | Negative | 0% | 0% | 0% | 0% | 10%-100% | 93.3% | 114.9% | 186.6% |
18 (1988) | Negative | 0% | 0% | 0% | 0% | Failed ampl | 0% | 0% | 0% |
19 (1988) | Negative | 1%-10% | 3.4% | 5.8% | 10.9% | Het met | 0.2% | 0.8% | 3.8% |
20 (1988) | Negative | 10%-100% | 12.5% | 14.4% | 21.8% | 0% | 0% | 0% | 0% |
21 (1988) | Positive | 0% | 0% | 0% | 0% | Het met | 0.1% | 0.5% | 2.4% |
22 (1988) | Positive | 0% | 0% | 0% | 0% | Het met | 2.2% | 2.7% | 3.8% |
23 (1988) | Negative | 10%-100% | 18.9% | 20.3% | 21.7% | 0% | 0% | 0% | 0% |
24 (1988) | Negative | 10%-100% | 9.5% | 14.4% | 15.4% | Het met | 0.1% | 0.2% | 0.7% |
25 (1988) | Negative | 10%-100% | 6.3% | 7.7% | 10.9% | 0% | 0% | 0% | 0% |
26 (1988) | Negative | 0% | 0% | 0% | 0% | Het met | 0.2% | 0.3% | 0.6% |
27 (1988) | Negative | 10%-100% | 12.5% | 14.4% | 15.4% | Failed ampl | 0% | 0% | 0% |
28 (1988) | Positive | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
29 (1993) | Negative | 1%-10% | 3.6% | 5.8% | 7.7% | 0% | 0% | 0% | 0% |
30 (1993) | Positive | 0% | 0% | 0% | 0% | 1%-10% | 2.9% | 8.8% | 14.4% |
31 (1993) | Negative | 0% | 0% | 0% | 0% | Het met | 0.3% | 0.7% | 1.4% |
32 (1993) | Negative | 10%-100% | 21.8% | 26.8% | 30.8% | 0.1%-1% | 0.1% | 0.3% | 0.7% |
33 (1993) | Negative | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
34 (1993) | Negative | 10%-100% | 25.0% | 40.6% | 61.6% | 1%-10% | 2.9% | 5.4% | 8.8% |
35 (1993) | Positive | 0% | 0% | 0% | 0% | Het met | 0.3% | 0.6% | 0.8% |
36 (1993) | Negative | 10%-100% | 5.1% | 10.9% | 17.7% | 0.1%-1% | 0.2% | 0.6% | 1.4% |
37 (1993) | Positive | 0% | 0% | 0% | 0% | 1%-10% | 23.3% | 25.0% | 28.7% |
38 (1993) | Positive | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
39 (1998) | Negative | 0.1%-1% | 0% | 0% | 0% | 0% | 2.5% | 2.9% | 3.4% |
40 (1998) | Negative | 0.1%-1% | 0.1% | 0.1% | 0.2% | Het met | 0.6% | 1.5% | 3.1% |
41 (1998) | Negative | 10%-100% | 19.0% | 23.3% | 28.7% | 0% | 0% | 0% | 0% |
42 (1998) | Positive | 0% | 0.1% | 0.3% | 0.7% | Het met | 0.2% | 0.4% | 1.4% |
43 (1998) | Positive | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
44 (1998) | Positive | 0.1%-1% | 0.1% | 0.2% | 0.4% | Het met | 2.9% | 3.6% | 5.4% |
45 (1998) | Positive | 0% | 0% | 0% | 0% | 0.1%-1% | 0.2% | 0.7% | 2.9% |
46 (1998) | Positive | 0% | 0% | 0% | 0% | 0.1%-1% | 0% | 0% | 0% |
47 (1998) | Positive | 0% | 0% | 0% | 0% | 10-100% | 21.8% | 30.8% | 50.0% |
48 (1998) | Positive | 0% | 0% | 0% | 0% | Failed ampl | 0% | 0% | 0% |
49 (2003) | Positive | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
50 (2003) | Positive | 0% | 0% | 0% | 0% | Het met | 0.5% | 1% | 2.1% |
51 (2003) | No data | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |
52 (2003) | Positive | 0.1%-1% | 0.4% | 0.6% | 1.0% | 1%-10% | 2.7% | 3.9% | 5.1% |
53 (2003) | Negative | 10%-100% | 2.7% | 6.3% | 16.5% | 1%-10% | 4.1% | 10.2% | 26.8% |
54 (2003) | Negative | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 0% |