Figure 4From: Secretion of MCP-1 and other paracrine factors in a novel tumor-bone coculture model Secretion of TGFβ1 and MMP2 in cultures. NCTC 2472 fibrosarcoma cells were cultured alone (Cells), neonatal femurs were cultured alone (BN) or these components were cultured together (Co-Cult) and media was collected at indicated times. Across four independent experiments, media was sampled from 8–10 wells for each time point, culture condition and media. A: Media concentration of transforming growth factor β1 (TGFβ1) at day 1 (white bar) and day 2 (black) are shown for both serum-free (left graph) and 1% horse serum (right graph) conditions. Data is graphed as mean pg/ml of TGFβ1 ± SE. B: Media concentrations of matrix metalloproteinase 2 (MMP2) at days and media conditions as in A. Data is graphed as mean (× 103) pg/ml of MMP2 ± SE. For A & B, significance of statistical comparisons between single-component cultures and cocultures where (a) means no significant difference whereas (b) means a significant difference at P < 0.001. Across three independent experiments, media was sampled from 5–6 wells for each time point, culture condition and media.Back to article page