Figure 4

Effects of ECRG4 overexpression or ECRG4 treatment in colorectal carcinoma cells on proliferation and viability. a) Reduced proliferation rate of ECRG4 transfected HCT116 and SW480 cells relative to control cells as determined by the BrdU assay (n = 3; p = 0.0133) (n = 3; p = 0.0775). b) Reduced cell viability of ECRG4 transfected HCT116 and SW480 cells relative to control cells as determined by the MTT assay (n = 4; p = 0.0005) (n = 3; p = 0.0003). c) Reduced cell viability of HCT116 and SW480 cells treated with ECRG4-containing medium relative to cells treated with medium from pCI Neo transfection as determined by the MTT assay (n = 3; p = 0.0603) (n = 3; p = 0.0397).