ECRG4 promoter methylation in primary glioma tissue samples. COBRA analysis of ECRG4 methylation in astrocytic gliomas of different WHO grades and two non-neoplastic brain samples (NB1, NB2). Representative data from 49 samples are shown. Arrows point to restriction fragments indicating a methylated sequence. The diagnosis is given in the subheadings. The tumor numbers are provided on top of each lane.