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Table 3 Nested PCR with the PGMY-GP system on previously negative or inconclusive results after primary PCR screen.

From: Sensitive HPV detection in oropharyngeal cancers

Sample

PGMY-GP nested PCR

HPV sequenced

Linear Array

C4

+

58

52m, 58

C5

+

87

-

V2post

-

ND

45, 54

V4on

+

16

54

V4post

+

16

16, 54

V6on

+

81

81

V6post

+

16

16

  1. Samples negative or weakly positive for HPV using any of the three PCR primer sets were re-amplified using a PGMY-GP5+/GP6+ nested approach. Positive samples were directly sequenced to determine HPV type and the result compared to Linear Array.
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BMC Cancer

ISSN: 1471-2407

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