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Table 3 Nested PCR with the PGMY-GP system on previously negative or inconclusive results after primary PCR screen.

From: Sensitive HPV detection in oropharyngeal cancers

Sample PGMY-GP nested PCR HPV sequenced Linear Array
C4 + 58 52m, 58
C5 + 87 -
V2post - ND 45, 54
V4on + 16 54
V4post + 16 16, 54
V6on + 81 81
V6post + 16 16
  1. Samples negative or weakly positive for HPV using any of the three PCR primer sets were re-amplified using a PGMY-GP5+/GP6+ nested approach. Positive samples were directly sequenced to determine HPV type and the result compared to Linear Array.