Figure 3

Isolation of mutations using digital HRM. Figure 3 shows sequence traces (3a) for genotypes identified by HRM (3b) following dilution of DNA from tumor 3641 and amplification from 6 pg DNA. Each melting curve is derived from a single amplicon. Three genotypes were identified in this tumor as shown in 3b and are depicted by red, blue and orange differential melting curves. Green melting curves represent that of wild-type, non-tumor control DNA. The wild-type genotype in sample 3641 has a G at nucleotide 157 (reverse sequence shown with nucleotide position denoted by *), and the mutant genotype has an A at this position. A sub-population with G/A was also identified.