Figure 3

Wild-type Aurora-A increases phosphorylation of MEK/ERK, AKT and the activity of RalA of Ras ^V12 transformants. (A) The phosphorylation of MEK was detected by anti-p-MEK antibody. The expression levels of AKT and p-AKT were detected using anti-AKT and anti-p-AKT specific antibodies.Ral A activity was detected by Ral pull-down assay. The expression level of each protein in three cells was shown graphically at the bottom panel. (B) After Aurora-A siRNA (5 μg) was transfected into WT cells for 6 h, IPTG was added and incubated for another 48 h. The total cell lysates (100 μg) were separated by 12% SDS-PAGE. Ral A, Ras, Aurora-A and β-actin were detected using Western blotting. The level of each protein was quantified by a densitometer. The expression level of each protein in Vector cells without IPTG induction was set as 1.0 fold. " * ": p ≦ 0.01