Detection of Ha- and Ki-RasG12V mutation and overexpression of Aurora-A in bladder and colon cancer specimens. (A) Sequence analysisof Ha-ras codon 12 mutations of bladder cancer specimen shows the Gly12 to Val12 mutation of Ha-ras gene. (B) Aurora-A protein expression in bladder cancer (T) and normal tissue (N) was detected by IHC staining using anti-Aurora-A specific antibody. (C) Sequence analysisof Ki-ras codon 12 mutations of colon cancer specimen shows the Gly12 to Val12 mutation of Ki-ras gene. (D) Aurora-A protein expression in colon cancer (T) and normal tissue (N). " * " indicates the mutation site of ras gene. The pink arrow points the expression of Aurora-A by IHC stain.