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Table 2 Primers and probes for TaqMan qRT-PCR

From: Inverse correlation between PDGFC expression and lymphocyte infiltration in human papillary thyroid carcinomas

Gene Sequence (5'-> 3')

Amplicon (bp)

Genebank Acc.No

PDGFA

  

   (+) TCGATGAGATGGAGGGTCG

71

NM_033023

   (-) ACCCGGACAGAAATCCAGTCT

  

   FAM-TGAGACCTCTGCACTTCCATCCCACG-TAMRA

  

PDGFB

  

   Applied Biosystems Assay On Demand, Hs00234042_m1

80

NM_033016.1

PDGFC

  

   (+) TATCTGATGAATATTTTCCTTCTGAACCAG

96

NM_016205

   (-) GTAGCACTGAAGGACTCACAGCTTCT

  

   FAM-TTCTGCATCCACTACAACATTGTCATGCCACAATTC-TAMRA

  

PDGFD

  

   Applied Biosystems Assay On Demand, Hs00228671_m1

73

NM_033135.3

PDGFRA

  

   (+) CAAGAGGAACAGACACAGCTCGCAGA

84

NM_006206

   (-) CTGCTGGAACCCGTCTCAAT

  

   FAM - CAAGAGGAACAGACACAGCTCGCAGA-TAMRA

  

PDGFRB

  

   Applied Biosystems Assay On Demand, Hs00182163_m1

86

NM_002609.3

LTB2

  

   Applied Biosystems Assay On Demand, Hs00242739_m1

78

NM_002341.1

IL2RG

  

   Applied Biosystems Assay On Demand, Hs00173950_m1

76

NM_000206.1

FYB

  

   Applied Biosystems Assay On Demand, Hs01061565_m1

123

NM_001465.3

IL32

  

   Applied Biosystems Assay On Demand, Hs00992441_m1

85

NM_004221.4

  1. Individual assays were either designed (PDGFA, PDGFC and PDGFRA) or ordered from Applied Biosystems, Assay On Demand, (PDGFB, PDGFD, PDGFRB, LTB2, IL2RG, FYB and IL32).
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BMC Cancer

ISSN: 1471-2407

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