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Table 4 Results of reconstruction experiments to optimise the immunobead RT-PCR method.

From: Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma

Marker Number of cells FaDu DETROIT
ESX 10 1 2
  50 2 3
  100 3 3
  500 3 3
CK19 10 2 2
  50 3 2
  100 2 3
  500 3 3
EGFR 10 0 0
  50 3 2
  100 2 2
  500 3 2
EphB4 10 0 0
  50 2 2
  100 3 1
  500 3 2
  1. Frequency of detection of known numbers of FaDu and Detroit cells (Number of cells) inoculated into normal peripheral blood samples using the immunobead RT-PCR method with specific markers ESX, CK19, EGFR and EphB4. Triplicate samples were processed and the number of samples positive with each marker in the two different cell lines is indicated.