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Table 3 Comparison of the attachment of immunobeads coated with antibodies BerEP4 and EMA to cells from four HNSCC cell lines.

From: Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma

  BerEP4 EMA
Cell line Cells with beads attached (%) Average number of beads attached/cell Cells with beads attached (%) Average number of beads attached/cell
FaDu 100 ± 0 6.4 ± 3.1 51.0 ± 5.0 2.1 ± 1.3
Detroit 98 ± 2 7.0 ± 3.9 13.7 ± 4.2 1.4 ± 0.7
SCC-9 100 ± 0 10.0 ± 2.0 61.5 ± 2.5 2.4 ± 1.1
SCC-25 100 ± 0 13.0 ± 4.0 30.0 ± 2.0 1.4 ± 0.5
  1. Cells were incubated with either BerEP4- or EMA-conjugated beads and a sample viewed using a light microscope. The BerEP4 antibody recognises the EpCam protein and the EMA antibody recognises Muc1/EMA/Mucin 1. The total number of cells and the number of these cells with beads attached were counted and the results are presented as a percentage of cells with beads attached. The average number of beads attached per cell was also determined.