Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma

BMC Cancer

Table 2 Primer pairs for amplification of marker gene sequences for RT-PCR expression and immunobead RT-PCR analyses.

Primer Name	Primer Sequence 5'-3'	Annealing Temperature	Product Size
ELF3 F	CTCGGAGCTCCCACTCCTCAGA	68°C	188 bp
ELF3 R	GCTCTTCTTGCCCTCGAGACAGT
EphB4 F	CCCCAGGGAAGAAGGAGAGCTG	68°C	251 bp
EphB4 R	GCCCACGAGCTGGATGACTGTG
EGFR F	TGTGAGGTGGTCCTTGGGAATTTGG	66°C	339 bp
EGFR R	TGCTGACTATGTCCCGCCACTGGA
CEA F	GGTTGGGGTTGCTCTGATATAGCAGC	68°C	97 bp
CEA R	GCTGTTGCAAATGCTTTAAGGAAGAAGC
CK19 F	GACTACAGCCACTACTACACGACC	68°C	743 bp
CK19 R	AGCCGCGACTTGATGTCCATGAGCC
EpCam F	GGACCTGACAGTAAATGGGGAAC	68°C	186 bp
EpCam R	CTCTTCTTTCTGGAAATAACCAGCAC

The sequences of forward (F) and reverse (R) primers for each of the markers are shown in 5' to 3' direction and were used at the indicated annealing temperature to generate a PCR product of the size indicated in base pairs (bp).

ISSN: 1471-2407