Localization of tyrosine phosphorylated MT1-MMP in NB cells. NB-10 neuroblastoma cells were transfected with either pcDNA, WT or Y573F forms of MT1-MMP. 24 h post-transfection, cells were seeded on 10 μg/ml type I collagen-coated cover slips, serum-starved for 18 h, stimulated for 15 min with serum and fixed with formaldehyde. Nuclei, actin and pMT1-MMP were stained as described in Material and Methods. Images were acquired using a Zeiss LSM 510 Meta confocal microscope. The white scale bar represents 50 μm.