VF conditioned medium induces intracellular lipid accumulation and cell polarity. (A) CM induces lipid accumulation and polarity in lipid distribution in M109 cells. White arrows mark M109 cells with polarized lipid distribution. CM was obtained by incubating 0.3 g VF in RPMI medium for 4 days. (B) CM induces polarity in the distribution of cytoplasmic GFP (leftmost panel), and surface proteins cadherin (middle panel) and vascular endothelial adhesion molecule I, VCAM-1 (rightmost panel). Cadherin and VCAM-1 are visualized by immuno-labelled with FITC conjugated antibodies. Two M109 cell lines were used: one cell line was stably transfected with plasmid expressing GFP, and the other untransfected cell line was used to stain for cadherin and VCAM-1 CARS: grey; TPEF: green. Scale bars: 25 μm.