Figure 4

Tem1/endosialin expression in mouse brain tumor models. A. Induction of Tem1/endosialin in intracranial U87MG xenografts grown in nude mice. ISH with mouse specific riboprobes detected Tem1/endosialin (Tem1) in tumor (T) but not normal mouse brain (NL). Probes for mouse vWF were used as a positive endothelial control (vWF). Original magnification, 200×. B. Survival of nude TEM1/endosialin WT and KO mice after intracranial injection with 10⁵ U87MG glioblastoma cells. Animals sacrificed after 100 days showed no evidence of tumor take. C. Tumor vessels stained for vWF in intracranial xenografts from nude TEM1/endosialin WT or KO mice. Size bar, 50 μm. D. Comparison of vessel numbers from nude TEM1/endosialin WT and KO tumors. Ten non-overlapping high power fields were counted per animal. Error bars, standard error of the mean. *p = 0.001.