Figure 1

Localization of TEM1/endosialin expression. A. Representative RT-PCR for TEM1/endosialin (TEM1) in whole cell lysates from two GBMs (T1, T2), two metastatic brain tumors (T3, T4) and two non-neoplastic controls (N1, N2). Expression was normalized to von Willebrand Factor (vWF), an endothelial positive control. B. Combined quantitative RT-PCR results for TEM1/endosialin in two controls (N), five GBMs (GBM) and five metastatic tumors (Met). Error bars, standard error of the mean. *p = 0.05. C. TEM1/endosialin was visualized by immunohistochemistry (IHC) using the TEM1/33 custom antibody or in situ hybridization (ISH) in glioblastoma (GBM), metastatic brain tumor (MET) and non-neoplastic brain tissues (Normal). Staining for vWF was used as an endothelial positive control. Original magnification for whole spot, 40×; detail, 100×.