Inhibition of Loxl2 expression in tumor cells induces transition from a mesenchymal to epithelial like morphology. A. The expression of Loxl2 in MDA-MB-231 and LM2-4 breast cancer cells and HT1080 fibrosarcoma cells was inhibited following infection with lentiviruses producing the Loxl2 targeting shRNA TRCN46195 (sh-195). Control cells were infected with a lentiviral vector carrying the control unrelated shRNA SHC002 (sh-c). Shown are western blots of cell lysates derived from control versus sh-195 infected cells probed with a polyclonal antibody directed against Loxl2 . B. Phase contrast microscopy pictures of cultures of HT1080, LM2-4 and MDA-MB-231 cells stably expressing the control shRNA SHC002 or the Loxl2 silencing shRNA TRCN46195.