Figure 6

A. MDA 231 cells treated with PDBD and subjected to NF-κB binding assay and NF-κB p65 activity was studied to determine DNA binding activity. B. MDA 231 cells were transiently transfected with NF-κB-luc, treated with varying concentrations of PDBD for 24 h and reporter studies were performed to determine NF-κB promoter activity. pRL-TK was used as the rennilla control plasmid. C. MDA 231 cells were treated with PDBD and lysates were subjected to either Western blot analysis (upper panel) or ELISA (lower panel) for expression profile of non-phosphorylated IκB-α. D. Nuclear and cytoplasmic fractions were obtained from MDA-231 cells, which were subjected to Western blot analysis using NF-κB p50, p55 and p65. β-actin was used as the loading control for cytoplasmic fraction and Histone H3 was used as the loading control for nuclear fraction.