Figure 4
From: Initial activation of EpCAM cleavage viacell-to-cell contact
Induction of EpCAM target genes. HCT8 (A) and MCF7 (B) cells were seeded at three different confluencies (D1-3). At day 1 and 2 cells were lysed and the protein expression of the EpCAM target genes c-myc, cyclin E and A, and efabp was assessed upon immunoblotting with specific antibodies. For a control, actin expression levels were assessed in parallel. Levels of target genes were assessed by image densitometry, normalised for the amount of actin, and values for D1 set to one for a reference. Target gene levels are given n-fold of D1. Shown are representative results from three independent experiments.