Figure 2
From: Initial activation of EpCAM cleavage viacell-to-cell contact
Visualisation of EpCAM cleavage dependence on cell-to-cell contact. (A) HCT8 cells were seeded at three different confluencies (D1-3), which resulted in single cells (D1), 20% (D2), and 80% confluency (D3). Thereafter, HCT8 cells were stained with antibodies specific for EpEX (green) and EpICD (red), and cellular DNA was stained with DAPI (blue). Merged pictures are displayed in the lower right quadrant of each section. Fluorescence was recorded with a laser scanning confocal microscope. Shown are three representative sections of each condition. (B) EpICD localisations were assessed semi-quantitatively and are given as percentages of cells with no (-), weak (+), intermediate (++), and strong (+++) staining at the membrane, at cell-to-cell contacts, and within the nucleus.