Figure 2

Western blot analysis of PADI4 expression in tumor cell lines, tumors and corresponding healthy tissues. Extracts were fractioned by electrophoresis and probed with anti-PADI4 antibody. PADI4 immunosignals (67 kDa) were detected in malignant tissues, tumor cell lines and some healthy tissues. Densities of the signals were significantly stronger in tumor tissues than in the corresponding healthy controls. Lanes 1–2, gastric adenocarcinomas and corresponding healthy tissues; lanes 3–4 lung adenocarcinomas and corresponding healthy tissues; lanes 5–6, hepatocellular carcinomas and corresponding healthy tissues; lanes 7–8, esophageal squamous cell cancers and corresponding healthy tissues; lanes 9–10, breast cancers and corresponding healthy tissues; lanes 11–12, breast fibroadenomas and corresponding healthy tissues; and lanes 13–15, A549, SKOV3 and U937 cells, respectively.