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Table 1 HER-2/neu amplification status by multiplex ligation-dependent probe amplification (MLPA) of 42 breast cancer patients in undissected sections and after manual and laser microdissection, in comparison with in situ hybridization and immunohistochemistry.
| MLPA | |||||||
|---|---|---|---|---|---|---|---|
| Case number | IHC | ISH | Undissected | Manual MD | Laser MD | DCIS | Tumor % |
| 1 | 0 | NA | 1 | 1 | 1 | Yes | 30 |
| 2 | 0 | NA | 1 | 1 | 1 | 30 | |
| 3 | 0 | NA | 1 | 1 | 1 | Yes | 10 |
| 4 | 2 | NA | 1 | 1 | 1 | 10 | |
| 5 | 2 | NA | 1 | 1 | 1 | Yes | 70 |
| 6 | 2 | NA | 1 | 1 | 2 | Yes | 70 |
| 7 | 2 | NA | 1 | 1 | 1 | 50 | |
| 8 | 2 | NA | 1 | 1 | 1 | 70 | |
| 9 | 2 | NA | 1 | 1 | 1 | 70 | |
| 10 | 2 | NA | 1 | 1 | 1 | 60 | |
| 11 | 2 | NA | 1 | 1 | 1 | 30 | |
| 12 | 2 | NA | 1 | 1 | 1 | 60 | |
| 13 | 1 | LA | 2 | 2 | 2 | 70 | |
| 14 | 2 | A | 2 | 3 | 3 | 80 | |
| 15 | 2 | A | 2 | 2 | 3 | Yes | 80 |
| 16 | 3 | A | 2 | 2 | 3 | 80 | |
| 17 | 2 | LA | 2 | 2 | 3 | 80 | |
| 18 | 0 | NA | 2 | 1 | 1 | Yes | 90 |
| 19 | 0 | NA | 2 | 1 | 1 | 80 | |
| 20 | 1 | A | 2 | 3 | 3 | 70 | |
| 21 | 1 | LA | 2 | 3 | 2 | Yes | 50 |
| 22 | 1 | NA | 2 | 2 | 1 | Yes | 80 |
| 23 | 1 | NA | 2 | 1 | 1 | Yes | 10 |
| 24 | 1 | NA | 2 | 1 | 1 | Yes | 60 |
| 25 | 0 | NA | 3 | 1 | 1 | 60 | |
| 26 | 0 | NA | 3 | 1 | 1 | 80 | |
| 27 | 1 | A | 3 | 3 | 3 | 70 | |
| 28 | 1 | NA | 3 | 3 | 3 | Yes | 30 |
| 29 | 3 | A | 3 | 3 | 3 | 50 | |
| 30 | 1 | A | 3 | 3 | 3 | 80 | |
| 31 | 2 | A | 3 | 3 | 3 | Yes | 70 |
| 32 | 3 | A | 3 | 3 | 3 | Yes | 80 |
| 33 | 3 | A | 3 | 3 | 3 | 20 | |
| 34 | 3 | A | 3 | 3 | 3 | 70 | |
| 35 | 3 | A | 3 | 3 | 3 | 80 | |
| 36 | 3 | A | 3 | 3 | 3 | 70 | |
| 37 | 3 | A | 3 | 3 | 3 | 30 | |
| 38 | 3 | A | 3 | 3 | 3 | 70 | |
| 39 | 3 | A | 3 | 3 | 3 | 60 | |
| 40 | 2 | A | 3 | 3 | 3 | Yes | 40 |
| 41 | 3 | A | 3 | 3 | 3 | Yes | 20 |
| 42 | 3 | A | 3 | 3 | 3 | 10 | |
