Figure 4
The effect of doxorubicin alone or nanoparticle loaded with doxorubicin on KHOS, KHOS R2 , U-2OS, andU-2OS R2 was analyzed (A-D). Varying concentrations ofdoxorubicin was added and consequently cultured for 5 days. Nanoparticle loaded with doxorubicin showed increasedanti-proliferative activity in both drug sensitive and resistantosteosarcoma cell lines in a dose dependent manner. Growth inhibition was assessed by MTT as described under Methods. The experiment wasrepeated four times in triplicate.