The effect of doxorubicin alone or nanoparticle loaded with doxorubicin on KHOS, KHOS
, U-2OS, andU-2OS
was analyzed (A-D). Varying concentrations ofdoxorubicin was added and consequently cultured for 5 days. Nanoparticle loaded with doxorubicin showed increasedanti-proliferative activity in both drug sensitive and resistantosteosarcoma cell lines in a dose dependent manner. Growth inhibition was assessed by MTT as described under Methods. The experiment wasrepeated four times in triplicate.