DNA damage in non-stimulated and PHA-stimulated blood cells caused by 2-h and 24-h etoposide treatment at the indicated doses detected by Comet Assay at pH = 12.3. A) 2-h treatment; B) 24-h treatment. Each bar represents the mean value of three independent experiments. Data were analyzed using the Mann-Whitney U test. (*= p < 0.05, *** = p < 0.001 vs. control).