Table 2 Details of the antibodies used for IHC.
Antigen | Source | Clone | Staining | Positive control | Dilution | Pre-incubation | Antigen Retrieval |
|---|---|---|---|---|---|---|---|
CA-RP X | Experimental (22) | MC | Cytoplasm Nuclear | Brain | 1:50 | Foetal calf serum 20 min | None |
SRD5A1 | Novus Biological | PC | Cytoplasm Nuclear | Prostate Carcinoma | 1:100 | None | Citrate buffer/10 min mwo |
ESR1 | Zymed | PC | Nuclear | Breast Carcinoma | 1:200 | Non-Fat Dry Milk 20 min | TrisEDTA/10 min mwo |
AR | Dako | AR441 | Nuclear | Cervix | 1:400 | none | TrisEDTA/10 min mwo |
CYP19 | Abcam | PC | Cytoplasm | Placenta | 1:300 | Non-Fat Dry Milk 20 min | Citrate buffer/10 min mwo |