Figure 2

SH122 promoted TRAIL-induced cell death in prostate cancer cell line DU145 (A), LNCaP (B) and CL1 (C). Cells (5,000 cells/well in 96-well plates) were treated with different concentrations of TRAIL and SH122, alone or in combination, with SH110 as a negative control. After incubation for 96 h, cells were stained with Cell Counting kit-8 reagent. The optical density of each sample was measured. Data were normalized as described in Materials and Methods. Data were presented as mean ± SD (n = 3).