Figure 1

Smac-mimetic compound interacted with XIAP and cIAP-1 in human prostate cancer cells. A. Structure of the Smac-mimetic compound SH122, and its inactive control compounds SH123 and SH110. B. Pull-down assay. Biotin-labeled SH122 (SH122BL, 20 μM) was incubated with whole cell lysates of DU145 cells with or without 10-fold excess of non-labeled SH122, followed by incubation with precleared Streptavidin agarose beads. Eluted beads were employed to Western blot analysis with anti-XIAP or cIAP-1 antibody. Data shown represent one of at least three independent experiments. SH122BL, Biotin-labeled SH122. C. CL1 cell lysate was incubated with 1 μM and 2.5 μM of SH122BL, or 2.5 μM of SH-123BL, with or without 10-fold excess of their unlabeled forms. SH123BL, Biotin-labeled SH123.