BrdU and TUNEL analysis after RNAi treatment. (a) BRAF knockdown using BRAF-C2 siRNA induces a significant (*p < 0.05) decrease in BrdU incorporation in comparison to the siRNA control in the three cell lines. (b) Inhibition of BRAF had no major effect on apoptosis as measured by TUNEL assay in 8505C and C643. In TPC1 cells there was a significant (*p < 0.05) increase in apoptosis when compared with the controls. All values presented are the mean of at least three independent experiments. In both, BrdU and TUNEL analysis, siRNA control did not produce any significant effect in comparison to the blank and transfection controls.