Immunization with ECD/Her2 induces strong antitumor immunity in mice. Groups of 9 mice were injected as described in Materials and Methods either with ECD/Her2 or with PBS on days 0, 21, and 42. 10 days after the last injection, mice were inoculated s.c. with 105 D2F2/E2 cells. This is one representative experiment of 3 performed. A. Kaplan-Meier curve was plotted for tumor free analysis. Unbroken line represents ECD/Her2 vaccinated mice and broken line PBS vaccinated mice (control group). B. Tumor growth in groups of vaccinated mice. The volume of individual tumors was calculated as (length × width2)/2. Curves represent individual mice. Black square represents ECD/Her2 vaccinated mice and open circle PBS vaccinated mice (control group). p < 0.01.