Knockdown of FZD7 reduces colony formation, but does not cause loss of POU5F1 expression in EC cells. NT2/D1-R1, 833K, and Tera-1 cells were each infected with lentiviral shRNAs to knockdown FZD7 expression. Graphs depict a representative experiment performed in triplicate, displayed as percent of control for each cell line. Error bars represent standard deviation. Real-time PCR assays were used to determine a) FZD7 gene expression and b) POU5F1 gene expression. c) Colony formation ability of FZD7 shRNA infected cells versus controls is displayed.