Over-expression of WNT7A promotes in vitro migration and invasion and in OVCAR-3 ovarian cancer cells. (A) Quantitative real-time reverse transcription-PCR detection of WNT7A mRNA in immortalized human ovarian surface epithelial cells (HOSE) and 11 ovarian cancer cell lines. All data was normalized to B2M expression. Data represents duplicate assays in duplicate experiments. (B) Western blot analysis confirmed higher levels of WNT7A in transfected clones (Wnt-C5 and Wnt-P2) as compared to the control clones (Con-P1 and Con-P2). (C) Migration ability was measured using an in vitro scratch wound healing assay. Above, photographs of wound regions taken immediately after or 24 hours after scratch was performed. Below, significant difference was confirmed between the WNT7A transfected clones and the mock clones upon measurement of the scratch wounds. (D) Invasion activity was measured in vitro using MICS invasion chambers; bars, SD. A dramatic increase in the number of invading cells was observed in both of the WNT7A transfected clones relative to the mock clones. bars, SD. *, p < 0.05; **, p < 0.02; ***, p < 0.01.