Figure 1

Prostasin IHC Staining in Bladder Tissues. The normal bladder tissue shown in A, B, C, and D, was part of a paraffin slide panel containing normal human tissues (Multi-Tissue VI) obtained from BioChain Institute, Inc. (Hayward, CA). The TCC and matching non-cancer tissues shown in E, F, G, and H, were part of a Bladder Carcinoma TMA (ARY-HH0087) obtained from Folio Biosciences (Columbus, OH). The ARY-HH0087 TMA contained 80 tissue cores, each at 1.5 mm in diameter; with 40 cancer tissues and 40 matching or independent non-cancerous tissues. The IHC staining with a prostasin-specific polyclonal antibody was carried out as described previously [11]. Prostasin-specific positive staining is shown by the intense brown color in A, B, C, E, and H. D. Normal w/o Pro Ab: IHC was performed with the pre-immune rabbit serum in place of the prostasin antiserum, serving as a negative control. Images were taken with various objective lenses (O.L.) as indicated in the figure and a SONY DXC-950 3CCD camera using a 0.45× coupler.