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Table 3 Fold expression of MSX2 mRNA after treatment with core thymic factors as analyzed by real-time PCR

From: NK-like homeodomain proteins activate NOTCH3-signaling in leukemic T-cells

Factor

JURKAT

MOLT-4

IL7

~ +/- 0.3

x7.5 +/- 1.5

BMP4

x0.5 +/- 0.2

x0.4 +/- 0.2

TGFbeta

nd

x3.0 +/- 0.3

anti-TGFBR2

~ +/- 0.2

x0.6 +/-0.2

sh-PU.1

nd

x0.7 +/- 0.1

sh-LEF1

nd

x3.0 +/- 1.5

sh-betaCatenin

nd

x0.01 +/- 0.1

anti-CD3

x0.5 +/-0.2

nd

Ionomycin

x0.5 +/-0.2

x0.1 +/-0.2

DAPT

x1.6 +/-0.2

 
  1. standard deviations (+/-), not determined (nd), nochange in expression (~)