Table 1 Oligonucleotides used for RT-PCR and ChIP
From: NK-like homeodomain proteins activate NOTCH3-signaling in leukemic T-cells
Gene | Acc.No | Forward primer (5'-3') | Reverse primer (5'-3') | PCR product (bp) |
|---|---|---|---|---|
HEX | NM_002729 | GCAAACCTCTACTCTGGAGC | TTCACTGGGCAAATCTTGCC | 311 |
HMX1 | NM_018942 | AGGCGGCCTCAGTCCTGACA | TGCGGGAGAAGACTGTGCGC | 263 |
HMX2 | XM_370580 | GCTTCACCATCCAGTCCATC | TTAAAGTCCGAGTGCGAAGG | 295 |
HMX3 | XM_291716 | TGGCTTTCCCTCGCTTTGAG | TCCTCCAGAATGATCTCGTC | 265 |
MSX1 | NM_002448 | AGAAGATGCGCTCGTCAAAG | ATCTTCAGCTTCTCCAGCTC | 339 |
MSX2 | XM_037646 | AGATGGAGCGGCGTGGATGC | ACTCTGCACGCTCTGCAATGG | 194 |
| Â | Â | GAATTCGAAGTCATGGCTTCTCCGTCC | TTGAATTCGGTGGTACATGCCATATCCC | 811 |
NKX1-1 | XM_926341 | AATCTGACAGGAGCGATTGG | TGGAACCAGATCTTCACCTG | 392 |
NKX1-2 | XM_372331 | TGGACCCACAGAAATTCACC | AACTTGTTCTCCAAGGCCAC | 460 |
NOTCH3 | NM_000435 | CCGCACCCAGCCTATTATTG | AGAAGTGGGAGGATCGCTTG | 219 |
| Â | Â | TAGACTGTCAGCTCCCTGAG | GCCCAGGAGTCTGAGGCTGC | 156 |
TEL | NM_001987 | AGGCCAATTGACAGCAACAC | TGCACATTATCCACGGATGG | 272 |