Protein interactions of NKL proteins. (A) Immunoprecipitation analysis (IP) has been performed for SPEN and TLE1, respectively, in JURKAT, PEER and ALL-SIL. Subsequent Western blot analysis (WB) indicates interaction with MSX2, NKX2-5 and TLX1, respectively. (B) Immunocytological analysis of MSX2 and SPEN was performed in JURKAT cells. DAPI staining (blue) illustrates the nucleus. Both, MSX2 (green) and SPEN (red) demonstrate a speckled distribution within the nucleus and large colocalizations (yellow).